Device

Part:BBa_J119458:Design

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2021-03-09)


tClone Blue: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1487
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849
    Illegal BsaI.rc site found at 42


Design Notes

None.


Source

Synthetic gene construction followed by ligation into pSB1A2-BR (BsaI site Removed).

References